Fig. 4. Effects of CXCR4 overexpression on the proliferation, migration and invasive ability in H1975 cell lines. A. CXCR4 was overexpressed in H1975 cells via an adenovirus carrying CXCR4. The mRNA levels were measured using qRT-PCR (n=3, #p<0.01 compared with negative control adenovirus transfection group). B. The CXCR4 protein levels were measured with Western blot in H1975 cells transfected with a CXCR4-carrying adenovirus (n=3, #p<0.01 compared with the negative control adenovirus transfection group). C. The migration ability of H1975 cells in the presence or absence of the CXCR4 adenovirus were measured with the wound-healing assay. Representative images were recorded at 0, 24 and 48 h post-wound time points. D. Quantification of healing distance (n=3, *p<0.05, compared with the adenovirus negative control group). E. Matrigel invasive assay of H1975 transfected with the CXCR4 adenovirus or control vector. Migratory cells were counted in 10 non-overlapping areas and quantified as shown in the bar graph (each group n=3, with 10 visions, *p<0.05, compared with the negative control adenovirus transfection group). F. H1975 cell proliferation was measured using a CCK-8 kit. The OD values were measured at 24, 48 and 72 h in the control group and the CXCR4 adenovirus transfection group (n=3, *p<0.01 compared with the negative control adenovirus transfection group).